- Transfection of Mammalian cells, selection of colonies, etc
- Molecular Biology: DNA/RNA purification, cloning (including vector and primer design), PCR, RT-PCR, restriction digests, preparation of competent cells, etc.
- Creation of retrovirus and transduction of insect cells
- Sucrose gradient isolation of lysosomal end ER membranes
- Radiolabelled peptide transport assays with analysis of transport kinetics
- Electrophoresis: SDS-PAGE, Agarose
- Blotting Techniques: Western, E.C.L.
- Cell Culture: Bacterial, Mammalian, and Insect